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PROTEIN ISOLATION AND IDENTIFICATION OF TRICHOSANTHES KIRILOWII SEED FROM DIFFERENT PLACES OF PRODUCTION

文献类型： 外文期刊

作者： Xie, Jin 1 ; Zhu, Xiaoqi 1 ; Song, Hong 1 ; Peng, Siwen 1 ; Huang, Yangning 1 ;

作者机构： 1.Hunan Acad Agr Sci, Inst Agroenvironm & Ecol, Changsha 410125, Hunan, Peoples R China

2.Key Lab Prevent Control & Remediat Soil Heavy Met, Changsha 410125, Hunan, Peoples R China

3.Changsha Med Plant Seedling Engn Technol Res Ctr, Changsha 410125, Hunan, Peoples R China

关键词： Trichosanthes kirilowii seed; Protien; Isolation and Identification

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）

ISSN： 0556-3321

年卷期： 2020 年 52 卷 1 期

页码：

收录情况： SCI

摘要： In this paper, 12 samples of Trichosanthes kirilowii seed from different places of production were studied. The total proteins of the 12 samples were extracted by Tris-phenol method. Twelve samples of proteins were isolated by SDS-PAGE. SDS-PAGE results showed that the protein bands in sample No.8 are different from those of other samples. And those protein bands were identified by mass spectrometry. At least 10 differentially expressed proteins were identified, including rRNA N-glycosidase, Acetyl-CoA acyltransferase, Citrate synthase, Phosphoglycerate kinase, Isocitrate dehydrogenase, Fructose-bisphosphate aldolase, Ferritin, Urease, Hhydroxyacyl-ACP-dehydratase, Glutathione peroxidase. From the result of protein identification, it can be inferred that the seed of No. 8 sample was superior to other samples during germination.

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