文献类型： 外文期刊

作者： Ding, Shan-Wen ¹ ; Wang, Dong-Wei ¹ ; Xiang, Yu ¹ ; Xu, Chun-Ling ¹ ; Xie, Hui ¹ ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Microbial Signals & Dis Co, Coll Agr, Dept Plant Pathol,Lab Plant Nematol, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Microbial Signals & Dis Co, Res Ctr Nematodes Plant Quarantine, Coll Agr,Dept Plant Pathol, Guangzhou 510642, Guangdong, Peoples R China

3.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China

关键词： Aphelenchoides ritzemabosi; fatty acid- and retinoid-binding protein; expression pattern; RNAi; reproduction; pathogenicity

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2019 年 20 卷 22 期

页码：

收录情况： SCI

摘要： The chrysanthemum foliar nematode (CFN), Aphelenchoides ritzemabosi, is a migratory, plant-parasitic nematode that is widely distributed and infects the aboveground parts of many plants. The fatty acid- and retinoid-binding proteins (FAR) are nematode-specific proteins that are involved in the development, reproduction, and infection of nematodes and are secreted into the tissues to disrupt the plant defense reaction. In this study, we obtained the full-length sequence of the FAR gene (Ar-far-1) from CFN, which is 727 bp and includes a 546 bp ORF that encodes 181 amino acids. Ar-FAR-1 from CFN has the highest sequence similarity to Ab-FAR-1 from A. besseyi, and they are located within the same branch of the phylogenetic tree. Fluorescence-based ligand-binding analysis confirmed that recombinant Ar-FAR-1 was bound to fatty acids and retinol. Ar-far-1 mRNA was expressed in the muscle layer, intestine, female genital system, and egg of CFN, and more highly expressed in females than in males among the four developmental stages of CFN. We demonstrated that the reproduction number and infection capacity of CFN decreased significantly when Ar-far-1 was effectively silenced by in vitro RNAi. Ar-far-1 plays an important role in the development, reproduction, infectivity, and pathogenesis of CFN and may be used as an effective target gene for the control of CFN. The results provide meaningful data about the parasitic and pathogenic genes of CFN to study the interaction mechanism between plant-parasitic nematodes and hosts.