您好，欢迎访问湖南省农业科学院 机构知识库！

Novel structural annotation and functional expression analysis of GTP_EFTU conserved genes in pepper based on the PacBio sequencing data

文献类型： 外文期刊

作者： Tang, Bingqian 1 ; Xie, Lingling 2 ; Li, Xuefeng 2 ; Yang, Huiping 1 ; Liu, Yi 1 ; Cheng, Xiang 1 ; Liang, Chenliang 2 ;

作者机构： 1.Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Hunan, Peoples R China

2.Hunan Acad Agr Sci, Vegetable Inst, Changsha 410125, Hunan, Peoples R China

3.Hunan Agr Univ, Coll Landscape & Hort, Changsha 410125, Hunan, Peoples R China

关键词： Capsicum; GTP_EFTU; PacBio; Phylogenetic analysis; Expression analysis; GO analysis

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：3.032； ）

ISSN： 2095-9885

年卷期： 2021 年 7 卷 5 期

页码：

收录情况： SCI

摘要： Genes containing GTP_EFTU domain mainly express elongation factors (EF), Small GTPases, and GTP-binding proteins, which are closely related to protein synthesis, extension and ATP synthesis. In this study, we identified 39 genes containing GTP_EFTU domains from peppers. The evolutionary trees constructed from capsicum, Arabidopsis, rice, and tomato are mainly divided into 7 subfamilies. Using PacBio (Pacific Biosciences) sequencing and assembly data, we extracted these 39 gene sequences, from which 25 genes had alternative splicing. Particularly, the Capana08g000545 had 16 alternative splicing processes. Accordingly, we performed promoter sequence analysis, subcellular location prediction, the expression analysis of different tissues and periods, and also the GO (Gene ontology) analysis of co-expressed genes. Lastly we did the qRTPCR analysis in 5 stages of pepper fruit development. These analyses revealed important structural and functional information for the identified 39 genes that contain GTP_EFTU domains, providing important references for further follow-up experiments to verify the genes function on plants or their unique roles in peppers.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台