您好，欢迎访问湖南省农业科学院 机构知识库！

Structural and biochemical basis of Arabidopsis FERONIA receptor kinase-mediated early signaling initiation

文献类型： 外文期刊

作者： Kong, Yanqiong 1 ; Chen, Jia 2 ; Jiang, Lingli 2 ; Chen, Hong 2 ; Shen, Yanan 2 ; Wang, Lifeng 3 ; Yan, Yujie 2 ; Zhou, Huan 4 ; Zheng, Heping 2 ; Yu, Feng 2 ; Ming, Zhenhua 1 ;

作者机构： 1.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Coll Life Sci & Technol, Guangxi Key Lab Sugarcane Biol, Nanning 530004, Peoples R China

2.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Hunan Key Lab Plant Funct Genom & Dev Regulat, Changsha 410082, Peoples R China

3.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

4.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai, Peoples R China

关键词： FERONIA; active conformation; kinase activity; autophosphorylation; activation

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2023 年 4 卷 4 期

页码：

收录情况： SCI

摘要： Accumulating evidence indicates that early and essential events for receptor-like kinase (RLK) function involve both autophosphorylation and substrate phosphorylation. However, the structural and biochemical basis for these events is largely unclear. Here, we used RLK FERONIA (FER) as a model and crystallized its core kinase domain (FER-KD) and two FER-KD mutants (K565R, S525A) in complexes with ATP/ADP and Mg2+ in the unphosphorylated state. Unphosphorylated FER-KD was found to adopt an unexpected active conformation in its crystal structure. Moreover, unphosphorylated FER-KD mutants with reduced (S525A) or no catalytic activity (K565R) also adopt similar active conformations. Biochemical studies revealed that FER-KD is a dual-specificity kinase, and its autophosphorylation is accomplished via an intermolecular mechanism. Further investigations confirmed that initiating substrate phosphorylation requires autophos-phorylation of the activation segment on T696, S701, and Y704. This study reveals the structural and biochemical basis for the activation and regulatory mechanism of FER, providing a paradigm for the early steps in RLK signaling initiation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台