Effects of different drying methods on the chemical constituents of Lilium lancifolium Thunb. based on UHPLC-MS analysis and antidepressant activity of the main chemical component regaloside A
文献类型： 外文期刊
作者： Yuan, Zhi-ying 1 ; Li, Zi-yi 1 ; Zhao, Hong-qing 1 ; Gao, Chuan 2 ; Xiao, Meng-wu 1 ; Jiang, Xing-ming 1 ; Zhu, Jian-p 1 ;
作者机构： 1.Hunan Univ Chinese Med, 301 Xueshi St, Changsha 410208, Hunan, Peoples R China
2.Hunan Agr Prod Proc Inst, Changsha, Peoples R China
3.Hunan Engn Technol Ctr Standardizat & Funct Chine, Changsha, Peoples R China
4.Key Lab TCM Heart & Lung Syndrome Differentiat &, Changsha, Peoples R China
关键词： antidepressant activity; drying methods; Lilium lancifolium Thunb; regaloside A
期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）
ISSN： 1615-9306
年卷期： 2021 年 44 卷 5 期
页码：
收录情况： SCI
摘要： The Lilium lancifolium Thunb. is a herb with multiple functions in both medicine and food in China, and its extracts have shown antidepressant effects. In this study, fresh bulbs of Lilium lancifolium Thunb. were processed to study the effects of different drying processes on changes in its main chemical components. We found that different drying methods can affect the chemical constituents of the herb. Among these components, Regaloside A has been found as the characteristic component. Here, Cell Counting Kit-8 assay, and Western blotting were used to evaluate the neuroprotective antidepressant effects of Regaloside A. The results showed the cell survival rate was improved, the phosphorylation levels of brain-derived neurotrophic factor, tyrosine kinase receptor B, phosphatidylinositol 3 kinase, protein kinase B, and mammalian target of rapamycin were increased after Regaloside A treatment. In general, different drying methods have a significant influence on the chemical composition of the herb, and Regaloside A may be the main chemical component of the herb. It can alleviate the damage of corticosterone in SH-SY5Y cells, and phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin signaling mediated by brain-derived neurotrophic factor/tyrosine kinase receptor B may play an important role in the neuroprotective antidepressant effects of Regaloside A.
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