文献类型： 外文期刊

作者： Zou, Zhaohui ¹ ; Li, Xian ¹ ; Deng, Gangqiao ¹ ; Xie, Hongke ¹ ; Zhang, Yong ¹ ; Liu, Jun ² ; Zhou, Yijie ¹ ; Tuand, Xiaoyi ¹ ; He, Aiguo ¹ ;

作者机构： 1.Hunan Acad Agr Sci, Changsha 410125, Peoples R China

2.Univ South China, Med Coll, Hengyang 421001, Peoples R China

3.Key Lab Hengyang City Ecol Impedance Technol Heav, Hengyang 421001, Peoples R China

关键词： Rice; C-13-N-15-glycine; Absorption and Transport; Mechanism

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：1.2； 五年影响因子：1.1 ）

ISSN： 0556-3321

年卷期： 2023 年 55 卷 1 期

页码：

收录情况： SCI

摘要： The study was conducted in sterile hydroponics using isotopes tracer technique to demonstrate the posibility for rice seedlings (variety: C Liangyou 266) to directly absorb and utilize molecular organic nitrogen, transport and assimilation of organic/inorganic nitrogen. Further the activity of assimilation-related enzymes i.e Glutamic-oxaloacetic transaminase (GOT), Glutamic-pyruvic transaminase (GPT) and Glutamate dehydrogenase (GDH), in rice seedlings were also studied using isotopic tracing technique. In this regard N-15 -ammonium sulfate, 15N -potassium nitrate and 2-C-13 -N-15 -glycine salts were used to analyzes the abundance of C-13/N-15. The results suggest that the 15N excess and the ratio of N-15 excess in shoot to root under glycine nitrogen treatment (Gly-N) were significantly higher than those under ammonium nitrogen or nitrate nitrogen treatment (p<0.05); the C-13 excess/N-15 excess ratios of rice organs and the whole seedling measured at 24h after Gly-N approximated the theoretical value of 1:1, which showed gradual declines 48h and 72h later. The activities of glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT) and glutamate dehydrogenase (GDH), in rice seedlings cultured by organic nitrogen were significantly higher than those by inorganic nitrogen (p<0.05). The findings also showed that rice seedlings are capable for direct absorbing and utilizing molecular glycine nitrogen. The absorption rate of glycine nitrogen was much higher than that of ammonium nitrogen or nitrate nitrogen. The transport capabilities of organic and inorganic nitrogen in rice plants, ranked in descending order are as follows: glycine nitrogen > ammonium nitrogen > nitrate nitrogen.