Vitamin B12-Induced Autophagy Alleviates High Glucose-Mediated Apoptosis of Islet β Cells
文献类型： 外文期刊
作者： Zhang, Yu 1 ; Chu, Ling 1 ; Zhou, Xi'an 1 ; Xu, Tingxia 1 ; Shen, Qingwu 1 ; Li, Tao 2 ; Wu, Yanyang 1 ;
作者机构： 1.Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Changsha 410128, Peoples R China
2.Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha 410125, Peoples R China
3.Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China
4.Hunan Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha 410128, Peoples R China
5.State Key Lab Subhlth Intervent Technol, Changsha 410128, Peoples R China
关键词： apoptosis; autophagy; diabetes; high glucose; vitamin B12
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 20 期
页码：
收录情况： SCI
摘要： High glucose levels can lead to the apoptosis of islet beta cells, while autophagy can provide cytoprotection and promote autophagic cell death. Vitamin B12, a water-soluble B vitamin, has been shown to regulate insulin secretion and increase insulin sensitivity. However, the precise mechanism of action remains unclear. In this study, we investigated the influence of vitamin B12 on high glucose-induced apoptosis and autophagy in RIN-m5F cells to elucidate how vitamin B12 modulates insulin release. Our results demonstrate that exposure to 45 mM glucose led to a significant increase in the apoptosis rate of RIN-m5F cells. The treatment with vitamin B12 reduced the apoptosis rate and increased the number of autophagosomes. Moreover, vitamin B12 increased the ratio of microtubule-associated protein 1 light chain 3 beta to microtubule-associated protein 1 light chain 3 alpha (LC3-II/LC3-I), while decreasing the amount of sequestosome 1 (p62) and inhibiting the phosphorylation of p70 ribosomal protein S6 kinase (p70S6K) under both normal- and high-glucose conditions. The additional experiments revealed that vitamin B12 inhibited high glucose-induced apoptosis. Notably, this protective effect was attenuated when the autophagy inhibitor 3-methyladenine was introduced. Our findings suggest that vitamin B12 protects islet beta cells against apoptosis induced by high glucose levels, possibly by inducing autophagy.
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