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Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop-P-methyl resistance in Digitaria sanguinalis

文献类型： 外文期刊

作者： Liu, Xiangying 1 ; Hou, Zhenlin 1 ; Zhang, Yuying 1 ; Merchant, Austin 2 ; Zhong, Mei-e 1 ; Ma, Guolan 2 ; Zeng, Qing 2 ; Wu, Lamei 1 ; Zhou, Xuguo 3 ; Luo, Kun 1 ; Ding, Chunxia 1 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China

2.Hunan Acad Agr Sci, Hunan Prov Key Lab Biol & Control Weeds, Changsha, Peoples R China

3.Univ Kentucky, Dept Entomol, Lexington, KY USA

4.Hunan Agr Univ, Sch Chem & Mat Sci, Changsha, Peoples R China

5.Univ Kentucky, S 225 Agr Sci Ctr North, Dept Entomol, Lexington, KY 40546 USA

关键词： Digitaria sanguinalis; metabolic resistance; haloxyfop-P-methyl; glutathione transferase; gene expression

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2023 年

页码：

收录情况： SCI

摘要： BackgroundHaloxyfop-P-methyl, an acetyl-CoA carboxylase (ACCase)-inhibiting herbicide, has been extensively used to control grass weeds. Widespread use of haloxyfop-P-methyl in cotton fields in China has led to the development of glutathione transferase (GST)-mediated resistance in Digitaria sanguinalis. An RNA-seq analysis identified DsGSTU1, a tau class glutathione transferase from the D. sanguinalis transcriptome as a potential candidate. Here, we cloned DsGSTU1 from D. sanguinalis young leaf tissues and subsequently characterized DsGSTU1 by a combination of sequence analysis, as well as functional heterologous expression in rice. RESULTSThe full-length coding DNA sequence (CDS) of DsGSTU1 is 717 bp in length. Higher DsGSTU1 expression was observed in haloxyfop-P-methyl-resistant (HR) D. sanguinalis than in haloxyfop-P-methyl-susceptible (HS) plants. Overexpression of the DsGSTU1 gene was confirmed by transformation into the wild-type (WT) Nipponbare rice with pBWA(V)HS, a recombinant expression vector. GST activity in transgenic rice seedlings was 1.18-1.40-fold higher than the WT rice seedlings before and after haloxyfop-P-methyl treatment, respectively. Additionally, transgenic rice seedlings overexpressing DsGSTU1 were less sensitive to haloxyfop-P-methyl. CONCLUSIONOur combined findings suggest that DsGSTU1 is involved in metabolic resistance to haloxyfop-P-methyl in D. sanguinalis. A better understanding of the major genes contributing to herbicide-resistant D. sanguinalis facilitates the development of resistance management strategies for this global invasive grass weed. & COPY; 2023 Society of Chemical Industry.

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