Genome-wide association study identified BnaPAP17 genes involved in exogenous ATP utilization and regulating phosphorous content in Brassica napus
文献类型： 外文期刊
作者： Li, Hao 1 ; Liu, Haijiang 3 ; Wang, Chuang 2 ; Zeng, Yang 1 ; Kant, Surya 4 ; Wang, Xiaohua 5 ; Hammond, John P. 6 ; Ding, Guangda 2 ; Cai, Hongmei 2 ; Wang, Sheliang 2 ; Xu, Fangsen 1 ; Zhang, Ying 7 ; Shi, Lei 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Microelement Res Ctr, Key Lab Arable Land Conservat Middle & Lower Reach, Minist Agr & Rural Affairs, Wuhan 430070, Peoples R China
3.Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China
4.La Trobe Univ, Sch Agr Biomed & Environm, AgriBio, 5 Ring Rd, Bundoora, Vic 3083, Australia
5.Linyi Univ, Coll Agr & Forestry Sci, Middle Shuangling Rd, Linyi 276000, Peoples R China
6.Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, England
7.Hunan Acad Agr Sci, Hunan Inst Agr Environm & Ecol, Changsha 410125, Hunan, Peoples R China
关键词：
期刊名称：PLANT CELL REPORTS （ 影响因子：4.5； 五年影响因子：6.1 ）
ISSN： 0721-7714
年卷期： 2024 年 43 卷 12 期
页码：
收录情况： SCI
摘要： Key messageBnaPAP17s associated with root-secreted APases activity were identified by genome-wide association study, and those were induced by Pi-deficiency. BnaPAP17s were involved in improving exogenous organophosphorus utilization as secreted APases.AbstractDeficiency of available phosphorus (P) in soil has become an important limiting factor for yield and quality in oilseed rape (Brassica napus). In many soils, organic P (Po) is the main component of the soil P pool. Po must be hydrolyzed to inorganic P (Pi) through acid Phosphatase (APases), and then taken up by plants. However, root-secreted APases (SAP) activity, as a quantitative trait, plays an important role in soil Po utilization; those genetic loci are not clear in B. napus. In this study, we performed a genome-wide association study for SAP activity under Pi-deficiency using a panel of 350 accessions of B. napus and more than 4.5 million polymorphic single nucleotide polymorphisms (SNPs). Thirty-five significant SNPs associated with SAP activity were identified. BnaA01.PAP17 (BnaA01g27810D) was a candidate gene underlying lead SNP (ChrA01_19576615). We experimentally verified that both BnaA01.PAP17 and its three homologous genes had similar expression pattern in response to Pi-deficiency. The dynamic changes in BnaPAP17s expression level were opposite to those of Pi concentration in both roots and leaves, suggesting their potential utility as Pi marker genes in B. napus. Transient expression of BnaPAP17s in tobacco leaves proved that BnaPAP17s were located in the apoplast as secreted APases. The overexpression of BnaPAP17s enhanced SAP activity in response to Pi-deficiency and resulting in increased P content in plants when ATP was supplied as the sole P resource. Taken together, these results suggest that BnaPAP17s contributed to SAP activity, thus having a function in extracellular Po utilization in B. napus.
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