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Combined Transcriptome and Metabolome Profiling Provide Insights into Cold Responses in Rapeseed (Brassica napus L.) Genotypes with Contrasting Cold-Stress Sensitivity

文献类型： 外文期刊

作者： Liu, Xinhong 1 ; Wei, Ran 1 ; Tian, Minyu 1 ; Liu, Jinchu 1 ; Ruan, Ying 1 ; Sun, Chuanxin 1 ; Liu, Chunlin 1 ;

作者机构： 1.Hunan Agr Univ, Key Lab Hunan Prov Crop Epigenet Regulat & Dev, Changsha 410128, Peoples R China

2.Hunan Acad Agr Sci, Crop Res Inst, Changsha 410125, Peoples R China

3.Hunan Agr Univ, Key Lab Crop Physiol & Mol Biol, Minist Educ, Changsha 410128, Peoples R China

关键词： cold-stress tolerance; ROS scavenging; silique length; sugar metabolism; ABA signaling; JA signaling; MAPK signaling; photosystems

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 21 期

页码：

收录情况： SCI

摘要： Low temperature is a major environmental factor, which limits rapeseed (Brassica napus L.) growth, development, and productivity. So far, the physiological and molecular mechanisms of rapeseed responses to cold stress are not fully understood. Here, we explored the transcriptome and metabolome profiles of two rapeseed genotypes with contrasting cold responses, i.e., XY15 (cold-sensitive) and GX74 (cold-tolerant). The global metabolome profiling detected 545 metabolites in siliques of both genotypes before (CK) and after cold-stress treatment (LW). The contents of several sugar metabolites were affected by cold stress with the most accumulated saccharides being 3-dehydro-L-threonic acid, D-xylonic acid, inositol, D-mannose, D-fructose, D-glucose, and L-glucose. A total of 1943 and 5239 differentially expressed genes were identified from the transcriptome sequencing in XY15CK_vs_XY15LW and GX74CK_vs_GX74LW, respectively. We observed that genes enriched in sugar metabolism and biosynthesis-related pathways, photosynthesis, reactive oxygen species scavenging, phytohormone, and MAPK signaling were highly expressed in GX74LW. In addition, several genes associated with cold-tolerance-related pathways, e.g., the CBF-COR pathway and MAPK signaling, were specifically expressed in GX74LW. Contrarily, genes in the above-mentioned pathways were mostly downregulated in XY15LW. Thus, our results indicate the involvement of these pathways in the differential cold-stress responses in XY15 and GX74.

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