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The unconventional resistance protein PTR recognizes the Magnaporthe oryzae effector AVR-Pita in an allele-specific manner

文献类型： 外文期刊

作者： Xiao, Gui 1 ; Laksanavilat, Nutthalak 3 ; Cesari, Stella 3 ; Lambou, Karine 3 ; Baudin, Mael 3 ; Jalilian, Ahmad 3 ; Telebanco-Yanoria, Mary Jeanie 2 ; Chalvon, Veronique 3 ; Meusnier, Isabelle 3 ; Fournier, Elisabeth 3 ; Tharreau, Didier 3 ; Zhou, Bo 2 ; Wu, Jun 1 ; Kroj, Thomas 3 ;

作者机构： 1.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Peoples R China

2.Int Rice Res Inst, Manila, Philippines

3.Univ Montpellier, PHIM Plant Hlth Inst, Inst Agro, IRD,INRAE,CIRAD, Montpellier, France

4.CIRAD, PHIM, Montpellier, France

5.Univ Angers, Inst Agro, INRAE, IRHS,SFR QUASAV, Angers, France

6.Ferdowsi Univ Mashhad, Fac Agr, Dept Biotechnol & Plant Breeding, Mashhad, Iran

期刊名称：NATURE PLANTS （ 影响因子：18.0； 五年影响因子：18.6 ）

ISSN： 2055-026X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating rice diseases. Disease resistance genes such as Pi-ta or Pi-ta2 are critical in protecting rice production from blast. Published work reports that Pi-ta codes for a nucleotide-binding and leucine-rich repeat domain protein (NLR) that recognizes the fungal protease-like effector AVR-Pita by direct binding. However, this model was challenged by the recent discovery that Pi-ta2 resistance, which also relies on AVR-Pita detection, is conferred by the unconventional resistance gene Ptr, which codes for a membrane protein with a cytoplasmic armadillo repeat domain. Here, using NLR Pi-ta and Ptr RNAi knockdown and CRISPR/Cas9 knockout mutant rice lines, we found that AVR-Pita recognition relies solely on Ptr and that the NLR Pi-ta has no role in it, indicating that it is not the Pi-ta resistance gene. Different alleles of Ptr confer different recognition specificities. The A allele of Ptr (PtrA) detects all natural sequence variants of the effector and confers Pi-ta2 resistance, while the B allele of Ptr (PtrB) recognizes a restricted set of AVR-Pita alleles and, thereby, confers Pi-ta resistance. Analysis of the natural diversity in AVR-Pita and of mutant and transgenic strains identified one specific polymorphism in the effector sequence that controls escape from PtrB-mediated resistance. Taken together, our work establishes that the M. oryzae effector AVR-Pita is detected in an allele-specific manner by the unconventional rice resistance protein Ptr and that the NLR Pi-ta has no function in Pi-ta resistance and the recognition of AVR-Pita.

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